Studies of T-284 and Sh-516 Cyanine Dyes as Fluorescent Probes for Specific Α-synuclein Nano-fibrils Detection
نویسندگان
چکیده
As continuation of previous studies, the ability of T-284 and SH-516 cyanine dyes (Figure 1) to selectively detect fibrillar proteins of various amino acids composition (insulin, lysozyme, αsynuclien) was evaluated using fluorescence spectroscopy methods (Table). Both dyes were shown to be applicable for detection of various fibrillar protein species (Figure 2). T-284 demonstrated particular sensitivity to wild-type and disease-related mutant A53T α-synuclein, while for SH-516 fluorescence response on proteins presence was almost the same (except for lysozyme). Addition of monomeric proteins hardly affected the emission intensities of the dyes.
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